

Once you're sure that both the ISO and your flash drive work properly, you can use the Ubuntu ISO Tool or just dd to burn the ISO to your flash drive. You can verify a good flash drive by running the read only check specified in the accepted answer to this question. Be sure that the results from the command you've run and that file (or below) are the exact same.

You can find the valid MD5 sums for 16.04.1 below, sourced from this file. You can remedy this particular issue by downloading a clean ISO from the official Ubuntu website, and then verifying the MD5 hash of the file using the below command: md5sum /path/to/the/ubuntu.iso More rarely, it can be a memory error, but this seems unlikely in your case. This issue is often caused by either a hardware failure with your drive (as suggested by Error 5, which is almost always an I/O error), or a problem with the disk image you are using.
